HEPATOPROTECTIVE ACTIVITY OF POLYHERBAL FORMULATION ENCOMPASSING ORIGINALTHERAPEUTIC PLANTS INRATS

Abstract

Rats and primary cultures of hepatocytes were used as the in vivo and in vitro models to evaluate the hepatoprotectiveactivity of the polyhedral formulation, comprising of Ajowan, Cardamom, Clove, Mace, Nutmeg. Carbon tetrachloride(CCl₄) was selected as hepatotoxin. Liv-52 was the reference hepatoprotective agent. In the in vivo study, serumtransaminase (SGOT and SGPT), alkaline phosphatase (ALP), total bilirubin, albumin together with total protein andhistopathological criteria for the evidences of liver injury. Carbon tetrachloride caused the alterations in all thebiochemicalparametersandcentrilobularnecrosis.Thesebiochemicalobservationsweresupplementedbyhistopathologicalexaminationofliversections.Variouspathologicalchangessuchasarchitecturalintactness,centrilobularobservedinratstreatedonlywithcarbontetrachloride,butthegroupstreatedwiththecarbontetrachloride and hepatoprotective formulations (Liv-52 and RVSPHF567) were protected to a moderate extent fromsuch pathological changes. It was concluded from the study that the prepared formulation has shown comparablehepatoprotectiveactivityagainstCarbontetrachlorideinducedhepatotoxicityinratsin comparisontoLiv-52.