GREEN SYNTHESIS OF SILVER NANOPARTICLES USING TAGETES ERECTA LEAVES AND INVESTIGATE ITS ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY

Abstract

A growing number of people are interested in the green synthesis of nanoparticles because of their potential uses in a variety of industries, such as agriculture and medical, and because they are environmentally benign. This work focuses on the environmentally friendly manufacture of silver nanoparticles (AgNPs) using the reducing and stabilising properties of Tagetes erecta leaves. Multiple techniques, including Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and UV-Vis spectroscopy, were used to characterise the synthesised AgNPs. In addition, the produced AgNPs' antibacterial and antioxidant properties were assessed. The outcomes showed that spherical AgNPs with an average size range of X to Y nm may be successfully synthesised. The presence of biomolecules from Tagetes erecta leaves involved in AgNP stabilisation and reduction was verified by FTIR analysis. AgNPs that were synthesised shown strong antibacterial action against a variety of harmful microorganisms, such as fungus and bacteria. Furthermore, as shown by their capacity to scavenge free radicals, the AgNPs showed notable antioxidant activity. In order to produce nanoparticles with promising antibacterial and antioxidant characteristics, Tagetes erecta-mediated AgNP synthesis offers a viable, ecologically friendly method that is also reasonably priced.

The aim of green synthesis of silver nanoparticles is the necessity to develop environmentally friendly processes that don't generate waste or dangerous, toxic solvents during their metabolic pathways. To do this, fresh Tageteserecta leaf extract that has undergone phytochemical screening was utilised to create nano silver particles. Next, an estimation of the extract's antioxidant and antibacterial capabilities was made. The extract's antimicrobial activity was assessed against Aspergillus niger, Actinomyces, Proteus vulgaris, Klebsiella, Candida krusei, and Streptococcus mutans. Finally, a comparison study was conducted to evaluate the antibacterial potential of an extract, silver nanoparticles, and common medications (ofloxacin and amphotericin-B).